Yamamoto S, Yoshimoto N, Tarmann C, Jungbauer A (2009) Binding site and elution behavior of DNA and other large biomolecules in monolithic anion-exchange chromatography. Ghanem A, Healey R, Adly FG (2013) Current trends in separation of plasmid DNA vaccines: a review. Liu MA (2011) DNA vaccines: an historical perspective and view to the future.
Pereira LR, Prazeres DMF, Mateus M (2010) Hydrophobic interaction membrane chromatography for plasmid DNA purification: design and optimization. Sousa A, Sousa F, Queiroz JA (2012) Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification. This behaviour can be linked to the presence of the more hydrophobic phenyl group in Capto Adhere, leading to stronger retention of ssDNA molecules, which have a more hydrophobic character due to a higher degree of base exposure. Another pronounced difference between the resins was observed in the inverted elution of ss- and dsDNA, where ssDNA eluted at 2.88 M NaCl on Capto Adhere, while on Capto Q ImpRes ssDNA eluted already at 1.47 M NaCl.
This recognition was not observed for Capto Adhere. Capto Q ImpRes provided a recognition for guanylate bases when samples of deoxynucleotides or poly(dG) were examined. All deoxynucleotides and DNAs tested bound strongly to the chromatographic materials and could be eluted by a linear gradient of increasing NaCl concentration. These variations in biophysical properties have been utilized for comparative separations on these two resins. The intrinsic differences between single- and double-stranded DNAs are related to charge, hydrophobicity, size and three-dimensional structure. Capto Adhere carries a multimodal ligand which combines strong anion with aromatic recognition, while Capto Q ImpRes is a strong anion exchanger with a chemically similar ligand, but without a phenyl group. Capto MMC ImpRes can typically be used in the initial polishing step in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs).The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Contaminants such as DNA, host cell proteins (HCP), leached protein A, and aggregates, are efficiently removed, making Capto MMC ImpRes an excellent choice for purification of MAbs and other biomolecules with high aggregate content. The multimodal functionality of Capto MMC ImpRes gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength.
Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity. 10 cm bed height of HiScreen columns allows method optimization and parameter screening. Displays a broad pH/salt operational window. Enables use of a platform approach to MAb process development. High yields achieved through the high-resolution beads and selectivity of the ligand. The columns are an excellent choice for method optimization and parameter screening. HiScreen Capto MMC ImpRes HiScreen Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto adhere MMC weak cation exchange multimodal medium.
The multimodal functionality gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength in combination with high resolution- and yield. The column is an excellent choice for method optimization and parameter screening. Ready to use ion exchange column, pre-packed with Capto adhere ImpRes multimodal medium. HiScreen Capto MMC ImpResHiScreen Capto MMC ImpRes
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